Journal: International Journal of Molecular Sciences

Article Title: Transcription Factor ZmNAC20 Improves Drought Resistance by Promoting Stomatal Closure and Activating Expression of Stress-Responsive Genes in Maize

doi: 10.3390/ijms24054712

Figure Lengend Snippet: Expression of ZmNAC20 in response to drought stress and ABA. ( A ) The relative expression level of ZmNAC20 in response to drought stress. ( B ) The relative expression level of ZmNAC20 after treatment with different concentrations of ABA. ( C ) The relative expression level of ZmNAC20 after treatment with 1 μM ABA for 0, 1, 2, and 3 h. ( D ) Relative expression of ZmNAC20 in ZmNAC20 -overexpressing transgenic plants. Asterisks indicate significant differences: ** p < 0.01 (Student’s t -test).

Article Snippet: ZmNAC20 ( Zm00001eb288360 in the version of Zm-B73-REFERENCE-NAM-5.0, Zm00001d038221 in version of Zm-B73-REFERENCE-GRAMENE-4.0 , GRMZM2G180328 in version of B73 RefGen_v3), which has been named in the previous study [ ], encodes a NAC-type transcription factor.

Techniques: Expressing, Transgenic Assay

Journal: International Journal of Molecular Sciences

Article Title: Transcription Factor ZmNAC20 Improves Drought Resistance by Promoting Stomatal Closure and Activating Expression of Stress-Responsive Genes in Maize

doi: 10.3390/ijms24054712

Figure Lengend Snippet: Phenotype of ZmNAC20 -overexpressing transgenic maize. ( A ) Drought resistance of transgenic maize overexpressing ZmNAC20 . Images were taken before and after drought stress treatment and rewatering. WT represented the wild-type B104 maize and served as the control. ZmNAC20-OE1 and ZmNAC20-OE2 represented the two ZmNAC20 -overexpressing maize lines. Bars, 5 cm. ( B ) Statistical data for the survival rate of WT, ZmNAC20-OE1 , and ZmNAC20-OE2 . The survival rate was obtained from 30 seedings in three independent tests. ( C ) The relative water content of maize leaves under drought stress treatment. The second leaves were cut from drought-treated plants. SE values were calculated from three biological replicates and more than 10 plants were examined in each replicate. ( D ) Fresh weight of maize leaves under drought stress treatment. SD values were calculated from three biological replicates and more than 10 plants were examined in each replicate. Asterisks indicate significant differences: ** p < 0.01 (Student’s t -test).

Article Snippet: ZmNAC20 ( Zm00001eb288360 in the version of Zm-B73-REFERENCE-NAM-5.0, Zm00001d038221 in version of Zm-B73-REFERENCE-GRAMENE-4.0 , GRMZM2G180328 in version of B73 RefGen_v3), which has been named in the previous study [ ], encodes a NAC-type transcription factor.

Techniques: Transgenic Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Transcription Factor ZmNAC20 Improves Drought Resistance by Promoting Stomatal Closure and Activating Expression of Stress-Responsive Genes in Maize

doi: 10.3390/ijms24054712

Figure Lengend Snippet: Stomatal aperture analyses of ZmNAC20 -overexpressing maize. ( A ) Statistics of relative water loss in detached leaves of WT and ZmNAC20-OE1 after dehydration for indicated times. WT represents the wild-type B104 inbred maize. ZmNAC20-OE1 represents ZmNAC20 -overexpressing transgenic maize. ( B ) Photograph of detached leaves after dehydration for 180 min. ( C ) Photograph of the stomatal aperture of detached leaves from WT and ZmNAC20-OE1 after ABA treatment. Bars, 10 μm ( D ) Statistics of the stomatal aperture of detached leaves after ABA treatment. Asterisks indicate significant differences, ** p < 0.01 (Student’s t -test).

Article Snippet: ZmNAC20 ( Zm00001eb288360 in the version of Zm-B73-REFERENCE-NAM-5.0, Zm00001d038221 in version of Zm-B73-REFERENCE-GRAMENE-4.0 , GRMZM2G180328 in version of B73 RefGen_v3), which has been named in the previous study [ ], encodes a NAC-type transcription factor.

Techniques: Transgenic Assay

Journal: International Journal of Molecular Sciences

Article Title: Transcription Factor ZmNAC20 Improves Drought Resistance by Promoting Stomatal Closure and Activating Expression of Stress-Responsive Genes in Maize

doi: 10.3390/ijms24054712

Figure Lengend Snippet: Subcellular localization of ZmNAC20. 35S:H2B-mCherry is a nuclear localization marker. Either 35S:ZmNAC20-GFP or the empty vector 35S:GFP and 35S:H2B-mCherry were co-transfected into leaves of Nicotiana benthamiana . The fluorescence of leaves was imaged using a confocal microscope. The merged signals of GFP and mCherry showed yellow color, suggesting ZmNAC20 was localized in the nucleus. Bars, 10 μm.

Article Snippet: ZmNAC20 ( Zm00001eb288360 in the version of Zm-B73-REFERENCE-NAM-5.0, Zm00001d038221 in version of Zm-B73-REFERENCE-GRAMENE-4.0 , GRMZM2G180328 in version of B73 RefGen_v3), which has been named in the previous study [ ], encodes a NAC-type transcription factor.

Techniques: Marker, Plasmid Preparation, Transfection, Fluorescence, Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Transcription Factor ZmNAC20 Improves Drought Resistance by Promoting Stomatal Closure and Activating Expression of Stress-Responsive Genes in Maize

doi: 10.3390/ijms24054712

Figure Lengend Snippet: ZmNAC20 regulates multiple signaling under drought stress by RNA-seq analyses. ( A ) Differential gene expression detected by DESeq2. A twofold change in gene expression levels between ZmNAC20-OE1 and wild-type B104 (WT) with a q-value (adjusted p -value) cutoff of 0.05 was considered as a differential expression. A total of 1361 genes were upregulated and 913 genes were downregulated in ZmNAC20-OE1 . ( B ) GO analyses of upregulated genes in ZmNAC20-OE1 . ( C ) KEGG analyses of upregulated genes in ZmNAC20-OE1 .

Article Snippet: ZmNAC20 ( Zm00001eb288360 in the version of Zm-B73-REFERENCE-NAM-5.0, Zm00001d038221 in version of Zm-B73-REFERENCE-GRAMENE-4.0 , GRMZM2G180328 in version of B73 RefGen_v3), which has been named in the previous study [ ], encodes a NAC-type transcription factor.

Techniques: RNA Sequencing, Gene Expression, Quantitative Proteomics

Journal: International Journal of Molecular Sciences

Article Title: Transcription Factor ZmNAC20 Improves Drought Resistance by Promoting Stomatal Closure and Activating Expression of Stress-Responsive Genes in Maize

doi: 10.3390/ijms24054712

Figure Lengend Snippet: ( A – I ) Expression of genes involved in drought stress response in ZmNAC20-overexpressing seedlings under drought stress. The seedlings were treated by dehydration for 3 h. SD values were calculated from three biological replicates Asterisks indicate significant differences: ** p < 0.01 (Student’s t -test), ns indicates no significance.

Article Snippet: ZmNAC20 ( Zm00001eb288360 in the version of Zm-B73-REFERENCE-NAM-5.0, Zm00001d038221 in version of Zm-B73-REFERENCE-GRAMENE-4.0 , GRMZM2G180328 in version of B73 RefGen_v3), which has been named in the previous study [ ], encodes a NAC-type transcription factor.

Techniques: Expressing

Journal: Plant Physiology

Article Title: Efficient Replication of the Plastid Genome Requires an Organellar Thymidine Kinase ^{1 [OPEN]}

doi: 10.1104/pp.18.00976

Figure Lengend Snippet: Albino phenotype and reduced cpDNA copy number in cptk1 plants. A, Pedigree showing the genealogical relationship between the assayed plants. B, Albino phenotype of homozygous cptk1 plants in two different inbred backgrounds. The diagram shows the cptk1 gene (GRMZM2G048821 in B73 genome v3) and the positions of the Mu insertion. The target site duplication is underlined. The nucleotide position with respect to the start codon is shown in parentheses. C, Quantification of mitochondrial and plastid DNA in cptk1 mutants. Each seedling is identified by the number of its family, whose relationships are diagrammed in A. Segments of the plastidial psbC and ndhA and mitochondrial atp6 and rps12 genes were assayed by qPCR. Results are the mean ± sd of technical triplicates and are represented as a ratio with respect to the wild-type (WT) in a log2 scale.

Article Snippet: An insertion in GRMZM2G048821 (B73 RefGen_v3) was found in all six of the mutant individuals shown in the pedigree in .

Techniques: